Fig. 4

EPO and AG490 administrations reduce the mRNA expression levels of inflammatory factors and increase the expression levels of antioxidants. Twenty-four hours after CLI, total RNA extracted from quadriceps was used for real-time reverse transcription polymerase chain reaction (RT-qPCR) to determine the mRNA expression levels of TNF-α, NF-kB, IL-10, HO-1, NQO-1, GPx and GR. a The mRNA expression levels of TNF-α. b The mRNA expression levels of NF-kB. c The mRNA expression levels of IL-10. d-g The mRNA expression levels of HO-1, NQO-1, GPx, and GR, four antioxidants. Statistical analysis used one-way ANOVA followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Symbols (*, †, ‡, §) indicate significance at p value less than 0.05