Fig. 1

EPO regulates the expression and phosphorylation levels of JAK/STATs in quadriceps after critical limb ischemia. a Twenty-four hours after CLI, total protein was extracted from quadriceps and Western blots were performed with antibodies against JAK2, phospho-JAK2, STAT5, phospho-STAT5, STAT1, phospho-STAT1, STAT3, and phospho-STAT3. b and c The expression levels of total and phosphorylated JAK2. d and e The expression levels of total and phosphorylated STAT1. f and g The expression levels of total and phosphorylated STAT3. h and i The expression levels of total and phosphorylated STAT5. Statistical analysis used one-way ANOVA followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Symbols (*, †, ‡,) indicate significance at p value less than 0.05. EPO, Erythropoietin; JAK, Janus kinase; STAT, Signal Transducer and Activator of Transcription