Fig. 7
Acetylation of histone 4 is enhanced in cells treated with H2S. Macrophages were stimulated either with a single dose of LPS 0.5 μg/ml (T group), a single dose of LPS 0.5 μg/ml followed by a higher dose of LPS (1 μg/ml) (TD group) or directly with LPS (1 μg/ml) (D group). Histone acetylation was measured at 4 (a) or 24 (b) hours. The H2S biosynthesis inhibitor AOAA and the H2S donor were both applied at 1 mM. *p < 0.05 TD vs. D groups; #p < 0.05 shows the inhibitory effect of AOAA, or the stimulatory effect of NaHS, compared to the respective group that did not receive any treatment with H2S modulators. From each experimental group, 3 samples were loaded on the Western blot gels, and the westerns were repeated 2 times. Bar values represent the mean ± SEM of the actin-corrected densitometry values. Western blot insets above the bars show representative acetylated H4 histone and actin bands