Fig. 1
Time course of MRI changes following intrastriatal microinjection of 100 ng/μl IL-1β. a Representative T 2 -w and T 1 -w images, ADC maps and their respective thresholded images from the same animal (1 SD below mean ADC of contralateral hemisphere of cohort imaged at 2.5 h, 6 h, 24 h and 72 h). Arrow points to injection site, visible as a focal area of T2-w hyperintensity at 2.5 h and 6 h, and reflects the volume of fluid injected. b ADC changes determined by thresholding. Mean ADC (error bars indicate a 1 SD) within the ipsilateral or contralateral striatum. Statistical significance indicated by *** p < 0.001, * p < 0.05; paired t-test, † p < 0.05; unpaired t-test and ▲▲▲ p < 0.001, ▲▲ p < 0.01; one-way ANOVA, post-testing using Bonferroni multiple comparisons test, with respect to the ADC of the IL-1β-injected hemisphere at 2.5 h c Evolution of area of reduced ADC. Mean area (error bars indicate a 1 SD) within the injected hemisphere. Statistical significance indicated by ▲ p < 0.05; one-way ANOVA, post-testing using Bonferroni multiple comparisons test, with respect to the area of reduced ADC at 6 h. d ADC measured within the dimensions of the 31 P MRS voxel (position and dimensions shown in Fig. 1a). Mean ADC (error bars indicate a 1 SD) within a region of interest encompassing the 31P MRS voxel within the ipsilateral or contralateral striatum. Statistical significance indicated by * p < 0.05; paired t-test ▲▲ p < 0.01; one-way ANOVA, post-testing using Bonferroni multiple comparisons test, with respect to the ADC of the IL-1β- injected hemisphere at 2.5 h. Vehicle- (saline) injected animals (n = 3 at each time point) and IL-1β-injected animals (n = 6 at each time point)