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Fig. 7 | Journal of Inflammation

Fig. 7

From: ERK2 and Akt are negative regulators of insulin and Tumor Necrosis Factor-α stimulated VCAM-1 expression in rat aorta endothelial cells

Fig. 7

RAEC transfected with shAkt and shERK2 exhibit increased insulin and TNFα-stimulated VCAM-1 at the cell surface as determined by flow cytometery. (Panel a) RAEC were stably transfected with shAkt (Akt KD) or (Panel b) shERK2 (ERK2 KD) and then transiently transfected with shERK2 or shAkt, respectively. Subsequently, cells were stimulated without or with insulin (10 nM) or TNFα (10 ng/mL) or in combination and surface VCAM-1 was determined by flow cytometry. C, non-transfected RAEC (no shRNA). NST, no stimulation. Akt KD, stable cell lines with shAkt. ERK2 KD, stable cell lines with shERK2. Akt KD + shERK2, stable shAkt cells lines transiently transfected with shERK2. ERK2 KD + shAkt, stable cell lines with shERK2 transiently transfected with shAkt. Surface VCAM-1 is expressed as percent of respective controls (not stimulated, No Stim) and represents the mean ± SEM of five independent experiments. *, P < 0.05 compared to their respective unstimulated controls. #, P < 0.05 compared to stimulated non-transfected controls

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