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Fig. 2 | Journal of Inflammation

Fig. 2

From: ERK2 and Akt are negative regulators of insulin and Tumor Necrosis Factor-α stimulated VCAM-1 expression in rat aorta endothelial cells

Fig. 2

Different clones of shRNA for Akt and ERK2 differentially downregulate their respective proteins. Akt and ERK2 shRNA clones provided by manufacturer were amplified in E. coli and isolated by plasmid preparation. Individual clones of each shRNA were transfected into different groups of RAEC as described in Methods. (Panel a) Akt and ERK2 proteins were quantified in unstimulated cells by Western blot analysis in untreated cells. “0” is negative control clone, and numbers 1–4 are different clones provide by the manufacturer. Intensity of bands are proportional to expression of designated protein in the presence of each shRNA clone. (Panel b) Relative protein expression is compared to controls (negative control clone)

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