Fig. 3
Influence of high concentrations of WIN55212-2 mesylate (WIN, 10−6M to 10−5M) and CB2 antagonist COR170 on TNF-induced IL-6 and IL-8 production in RA and OA synovial fibroblasts. a, b Modulation of WIN effects by CB2 antagonism on TNF-induced IL-6 and IL-8 production. c, d Impact of CB2 antagonist COR170 alone on IL-6 and IL-8 production in RASFs and OASFs. Cytokine production was determined under hypoxic (1 % O2) conditions. *p < 0.05 for comparisons vs control (TNF only, 100 %, dotted line). For comparisons versus control (100 %) the paired t-test was used; for comparisons between OA and RA (c, d), the t-test was used. The general linear model with Bonferroni correction was used for comparisons between WIN only and WIN together with CB2 antagonist (a, b). Data are depicted as mean ± sem. Corresponding p-values for comparisons are given in the graph. Data in C) and D) are visualized as box plots/vertical dot blots. The boundary of the box closest to zero indicates the 25th percentile, the line within each box indicates the median and the upper boundary of the box the 75th percentile. Error bars below and above the box indicate the 10th and 90th percentiles