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Fig. 3 | Journal of Inflammation

Fig. 3

From: Role of goblet cell protein CLCA1 in murine DSS colitis

Fig. 3

Increase in Cxcl-1- and Il-17-mRNA expression in Clca1 -/- mice during DSS-challenge. During DSS colitis, a Cxcl-1, b Cxcl-2, c Il-17, d Tnf and e Ifnγ were upregulated in the distal colon as the prime target site of DSS and sporadically also in the proximal colon. However, only Cxcl-1 and Il-17 showed a greater than two-fold increase of mRNA in the distal colon of Clca1 -/- compared to WT. At earlier time points, Cxcl-2 was upregulated at 24 h in Clca1 -/- proximal colon and at 48 h in all except for WT distal colon. At 48 h, Cxcl-1, Tnf and Ifnγ were upregulated in proximal colon of Clca1 -/- and WT, in proximal and in distal colon of WT mice, respectively. Ifnγ showed a more than five-fold decrease in the distal colon of DSS-challenged Clca1 -/- compared to WT at 24 and also at 48 h. Expression of f Tff3 in distal Clca1 -/- and WT colon and of g E-cad in distal Clca1 -/- colon was lower during colitis conditions, however, without difference between the genotypes. Dotted lines indicate a fold change of 0.5 and 2, respectively, as limits for valid statement of lowered or elevated expressions. A greater than two-fold difference in fold change was considered as relevant difference between the genotypes. Ct, cycle threshold. Relative quantification and comparison of groups were performed by the ∆ΔCt method using unchallenged WT animals as controls (fold change = 1). n = 9–18 per group

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