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Fig. 3 | Journal of Inflammation

Fig. 3

From: Nur77-mediated TRAF6 signalling protects against LPS-induced sepsis in mice

Fig. 3

Nur77 inhibits inflammatory response in in vivo mouse model of sepsis by interacting with TRAF6 and regulating TRAF6 auto-ubiquitination (a) Immunoblot analysis of indicated signalling proteins in liver (left) and spleen (right) from Nur77+/+ and Nur77−/− mice 2 h after LPS (20 mg/kg) challenge. b Immunoprecipitation and immunoblot of liver and spleen proteins from C57BL/6 mice 1 h after LPS (20 mg/kg) treatment. c Immunoprecipitation of endogenous TRAF6 from liver (left) and spleen (right) lysates of LPS-treated Nur77+/+ and Nur77−/− mice, and immunoblot of TRAF6 auto-ubiquitination with anti-ubiquitin antibody. d Immunoprecipitation of endogenous TRAF6 from lysates of LPS-treated (50 ng/ml) RAW264.7 cells expressing vector or myc-tagged Nur77 plasmid and immunoblotted for TRAF6 auto-ubiquitination with an anti-ubiquitin antibody

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