Fig. 2

Effect of CFTR knockdown and activation on IL-6, −8 and −10 secretion in Caco-2/15 cells. CFTR knockdown or control (Scrambled) cells were incubated 24 h in the absence (a) or presence (b, d, f) of 25 ng/mL of either TNF or IL1-β. (c, e) TNF or IL1-β-stimulated interleukin secretion following pharmacological activation of CFTR. Prior to incubation with TNF or IL1-β, cells with intact CFTR were treated with a mixture of 10 μM forskolin and 100 μM IBMX for 24 h. Supernatants were then collected to quantify the secretion of IL-8 (a-c), −6 (d-e) and −10 (f) by ELISA. Results represent the means ± SEM of n = 3–8 independent experiments and are illustrated as pg/mL normalized to total protein concentration. *p <0.05 and #p <0.0001 vs. scrambled-infected cells