Fig. 1

CFTR knockdown in Caco-2/15 cells. Caco-2/15 cells were infected with a lentiviral vector carrying either a scrambled sequence or shRNAi against CFTR and analyzed for gene (a) and protein (b) expression of CFTR by Q-PCR and Western blotting when they reached 80 to 90 % of confluence. Results represent the means ± SEM of independent experiments and are illustrated as % of controls after calculating the data as densitometric ratios of CFTR to the housekeeping gene RPL27 for gene expression (n = 10) or CFTR to β-actin for protein expression (n = 4). *p < 0.05 vs. scrambled-infected cells