Fig. 5
From: Nitric oxide secretion in human conjunctival fibroblasts is inhibited by alpha linolenic acid
Inhibition of NOS-2 expression in inflammatory-induced HCF cells by Alpha-linoleic acid (ALA). Cultures of HCF cells were stimulated using combination 1 (Table 1) for 72 h treatment and NOS-2 mRNA levels were measured using Real-time PCR (n = 4). Each bar represents the mean SD of NOS-2 expression in quadruplicate cell cultures. The asterisk (P < 0.01) represents statistical significance for HCF cells stimulated using combination 1 after ALA treatment vs HCF cells stimulated using combination 1. Double asterisk (P < 0.001) represents statistical significance for HCF cells stimulated with combination 1 vs HCF cells in medium alone. LPS - Lipopolysaccharide, ALA - alpha-linoleic acid; HCF – human conjunctival fibroblasts; NOS-2 - nitric oxide synthase 2