Fig. 4

n-PG inhibits RLR signaling in primary astrocytes. a Bar-graph showing the results of a cell reporter assay for the activation of RLR signaling activation/production of IFN-α/β following stimulation of primary rat astrocytes with the RLR ligand poly(I:C)LMW at a concentration of 1, 3 or 6 μg/ml for 18 h. C = control (untreated cells). b Bar-graph showing the results of a cell reporter assay for the activation of RLR signaling activation/production of IFN-α/β following stimulation of primary rat astrocytes with the RLR ligand poly(I:C)LMW at a concentration of 6 μg/ml and treated with n-PG at a concentration of 5, 10, 50 μM for 18 h. C = control (untreated cells). c Representative immunoblot analysis of astrocyte lysates of cells in culture subjected to OGD (4 h) and astrocytes pretreated with 50 μM n-PG for 2 h and then subjected to OGD. Cell lysates were immunoblotted with an antibody against IFN-α. β-Actin was used as a standard and control for protein loading. Data presented as mean+/−SEM. *p < 0.05. N = 6 OGD and 8 OGD + nPG