Fig. 3
From: RIG-I contributes to the innate immune response after cerebral ischemia
OGD activates RLR signaling in primary astrocytes in culture. Representative immunoblot analysis of astrocyte lysates of control (c) and astrocytes subjected to OGD and then harvested at 2 h, 3 h and 4 h (a). Cell lysates were immunoblotted with antibodies against (b) RIG-I and (c) IFN-α. β-Actin was used as a standard and control for protein loading. d Bar graph showing NF-κB activity following OGD in astrocytes. Data presented as mean+/−SEM. *p < 0.05. N = 6 per group