Fig. 7

EGCG induces IDO-dependent Nrf-2 activation in joints of CIA mice. a Representative western blots for Nrf-2, phosphorylated Nrf-2 (pNRF2), and HO-1 expression in joint homogenates. b Densitometry results for pNrf2, Nrf2 and HO-1 as shown in (a) as relative fold change compared to the GAPDH loading control. Results are the mean ± SD of replicate samples, representative of three independent experiments. c Representative western blots for Nrf-2, pNRF2, and HO-1 expression from joint homogenates of vehicle-fed, EGCG-fed or EGCG-fed + 1-MT treated CIA mice. d Densitometry results for pNrf2, Nrf2 and HO-1 are shown for (c) as relative fold change compared to the GAPDH loading control. Results are representative of three independent experiments, with 4 to 5 mice per group. e Immunohistochemical staining, original magnification, X200, for pNrf2 in knee sections from vehicle-fed, EGCG-fed or EGCG-fed + 1-MT treated CIA mice (upper panel). Cartilage degradation was revealed by safranin-O staining of paw sections from vehicle-fed, EGCG-fed or EGCG-fed + 1-MT treated CIA mice (lower panel). Results are representative of three independent experiments