Fig. 4
EGCG treatment attenuates T cell proliferation in CIA. Cells from the dLN of CIA mice sacrificed seven weeks after CIA induction were cultured for 72 h in the absence or presence of a 10 ug/ml CII or b anti-CD3 antibody. After pulsing with 1 uCi of [3H] thymidine per well for the last 18 h, proliferation was measured as [3H] thymidine incorporation in counts per minute (cpm). Results shown are representative of data from 4 to 5 mice per group from three independent experiments with similar numbers of mice; ∗∗∗P < 0.001. c,d 5,6-carboxyfluorescein succinimidyl ester (CFSE)-labeled dLN cells were cultured with type II collagen for 72 h, followed by flow cytometric analysis of CD4+ T cells; ∗P < 0.05. Data are representative of one sample (histograms) and one experiment (graph) from three independent experiments with 4 to 5 mice per group per experiment