Skip to main content
Fig. 2 | Journal of Inflammation

Fig. 2

From: Green Tea Epigallocatechin-3-Gallate Suppresses Autoimmune Arthritis Through Indoleamine-2,3-Dioxygenase Expressing Dendritic Cells and the Nuclear Factor, Erythroid 2-Like 2 Antioxidant Pathway

Fig. 2

Proinflammatory cytokine expression in EGCG-fed CIA mice. Changes in the levels of IL-1β, IL-6, TNF-α, IFN-γ and IL-10 were measured in a sera and b joint homogenates using ELISA assays and joint samples were normalized by protein concentration. All data are shown as mean ± SEM from three independent experiments involving 4 to 5 mice per group (*P < 0.05; **P < 0.01; ***P < 0.001). c,d The draining lymph node cells from CIA mice treated with EGCG or vehicle were cultured with PMA (25 ng/ml) and ionomycin (250 ng/ml) in the presence of a protein export inhibitor for 5 h. Frequencies of TNF-α and IFN-γ expressing CD4+ T cells were assessed by intracellular staining and flow cytometric analysis. Frequencies of TNF-α and IFN-γ were significantly reduced in the EGCG-fed mice as compared to controls whereas frequencies of TNF-α+ IFN-γ+ cells were similar between the two groups. The results shown were from one representative experiment of three independent experiments

Back to article page