Fig. 5

Effect of EF24 on the membrane integrity, proliferation, and cell cycle of JAWS II DCs. The cells were exposed to EF24 in the concentration range of 0.1-50 μM for up to 48 h. Membrane integrity was measured by (a) LDH activity in culture medium, whereas cell proliferation was quantified by (b) hexosaminidase enzyme assay. All the data (mean ± sem) are presented as percent of control (dotted line). (c) Flow cytometric analyses of cell cycle in JAWS II DCs treated with EF24 (1 and 10 μM). Propidium iodide staining was used to measure the DNA content of DCs after 24 and 48 h of EF24 exposure