Fig. 1

EF24 reduces LPS-induced TLR4 expression in JAWS II DCs. The DCs were treated with 100 ng/ml LPS for 4 h, followed by 1 h treatment with 1 or 10 μM EF24. (a) TLR4 mRNA expression (mean ± sem) in DCs treated with EF24. (b) TLR4 immunoblot of DCs lysates. The cells were treated with EF24 either after (↓) or before (↑) LPS addition as described in the material and methods. # p value < 0.05 vs. control and * p value < 0.05 vs. LPS treatment