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Fig. 2 | Journal of Inflammation

Fig. 2

From: Serum biomarkers VEGF-C and IL-6 are associated with severe human Peripheral Artery Stenosis

Fig. 2

Blood monocytes in PAS patients produce inflammatory cytokines and VEGF-C. a PBMCs were isolated from blood of 8 healthy subjects and 9 PAS patients by density-gradient centrifugation with Ficoll (1.077), followed by staining with FITC-conjugated anti-CD11b and APC-conjugated anti-F4/80 for flow cytometer analysis. b Relative mRNA levels IL-6, VEGF-C or IL-1β in PBMCs from healthy subjects and PAS patients were tested with qRT-PCR. c Freshly isolated monocytes from healthy subjects and PAS patients were either untreated (n = 6) or stimulated with different doses of LPS for 24 h (n = 10), followed by detection of IL-6 and VEGF-C concentrations from the supernatants by ELISA. Data presented are representative of three replicated experiments

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