Fig. 6

p38 MAPK activity is regulated by Zfp36l1 and Zfp36l2 through Mkp-1. a Basal activities of three MAPKs in Zfp36l1- and Zfp36l2-knockdown cells. Whole-cell extracts were isolated from RAW264.7 cells with Zfp36l1, Zfp36l2, or Zfp36l1/Zfp36l2 knockdown as indicated. Western blotting was performed to detect MAPKs and phospho-MAPKs. The asterisk indicates cross-reacted Mkp-2. b Mkp-1 expression and p38 activation profiles in different knockdown cells stimulated with LPS for 0, 15, 30, 45, or 60 min. Whole-cell extracts were collected and separated by SDS-PAGE, and analyzed by western blotting with anti-Mkp-1, anti-p-38, anti-p38, and anti-β-tubulin antibodies. The asterisk indicates cross-reacted Mkp-2. All of experiments were performed at least three times, and representative data were displayed. c The relative expression levels of Ttp and Tnfα mRNAs in different knockdown cells after LPS stimulation for 15 min. RNA was isolated and performed real-time PCR analysis