Fig. 5
Induction of Mkp-1 mRNA early during LPS stimulation is post-transcriptionally modulated by Zfp36l1 and Zfp36l2. a mRNA expression profile of Mkp-1 in RAW264.7 cells stimulated with LPS for 0, 15, 30, 45, 60, or 120 min. b Levels of Mkp-1 mRNA in different knockdown cells after LPS stimulation for 15 min. RNA was isolated and performed the real-time PCR analysis. c Analysis of Mkp-1 mRNA half-life in different knockdown cells after LPS stimulation for 15 min. Actinomycin D (10 μg · mL-1) was added to stop transcription after 0, 10, or 20 min. The remaining mRNA was detected by quantitative PCR. Mkp-1 mRNA half-life was calculated by exponential regression, 32 min, 55 min, 68 min, and 42 min in control, Zfp36l1, Zfp36l2, and dual-knockdown cells, respectively. All of experiments were carried out at least three times