Fig. 1

Consistent expression and phosphorylation of Zfp36l1 and Zfp36l2 during LPS stimulation in RAW264.7 cells. a Protein expression profiles of TTP family members in RAW264.7 cells stimulated with LPS for 0, 15, 30, 60, or 120 min. Cytoplasmic extracts (CE) and nuclear extracts (NE) were isolated for western blotting analysis using the indicated antibodies. β-tubulin was a loading control for the cytoplasmic extract, and hnRNPC1/C2 was a loading control for the nuclear extract. Positions of molecular size markers (M) are shown to the right. The blots were performed at least three times, and representative results from replicate experiments were presented. b mRNA expression profiles of TTP family members in RAW264.7 cells stimulated with LPS for 0, 15, 30, 60, or 120 min. RNA was isolated for quantitative PCR analysis to measure Ttp, Zfp36l1, Zfp36l2 mRNA levels. The relative mRNA level was shown as mean ± SD of three independent samples normalized to β-actin mRNA level. c Cytosolic extracts from RAW264.7 cells stimulated with LPS for 120 min were treated with calf intestinal phosphatase for 30 min and then were subjected to SDS-PAGE for western blotting analysis