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Fig. 3 | Journal of Inflammation

Fig. 3

From: Δ9-Tetrahydrocannabinol (THC) enhances lipopolysaccharide-stimulated tissue factor in human monocytes and monocyte-derived microvesicles

Fig. 3

No effect of THC on selected LPS-stimulated signal transduction molecules. Monocytes were stimulated with 30 μM THC or vehicle 30 min prior to 100 ng/mL LPS for indicated time periods. a Whole cell lysates were subjected to western blot for indicated proteins. Representative blots of one donor are shown. Similar results were obtained in 3 donors. Phospho-ERK1/2(p-ERK1/2) blot was stripped and reprobed for total ERK1/2. Phospho-p65 (p-p65) blot was stripped and reprobed for total p65. IκBα blot was stripped and reprobed for actin. Densitometry of data from 3 donors is graphed for pERK1/2: total ERK1/2 ratio (b), p-p65: total p65 ratio (c), and IκB: actin ratio (d). Graphs are normalized to untreated samples and depict mean and standard error for 3 donors

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