Fig. 2

THC has dose dependent effects on TF expression and activity. Monocytes were treated with THC at indicated concentrations or vehicle control for 30 min prior to the addition of 100 ng/mL LPS for 24 h. a Whole cell lysates were analyzed by western blot for indicated proteins. TF western blots were stripped and reprobed for actin. b Densitometry showing ratio of TF to actin across 5 donors. c Monocyte TF activity was measured. d, e Microvesicles were prepared from supernatants from monocytes treated as in a-c and measured for d microvesicle TF activity or e counted by flow cytometry. b-e Panels represent at least 5 donors and data are expressed relative to LPS only treatment (grey bar). Graphs show mean and standard error. * p < 0.05, ** p < 0.01 via ANOVA followed by Bonferroni post test