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Fig. 1 | Journal of Inflammation

Fig. 1

From: Δ9-Tetrahydrocannabinol (THC) enhances lipopolysaccharide-stimulated tissue factor in human monocytes and monocyte-derived microvesicles

Fig. 1

THC prolongs TF expression and procoagulant activity. Monocytes were treated with 30 μM THC or vehicle control for 30 min prior to the addition of LPS (100 ng/mL) for indicated time periods. TF activity in (a) monocytes or (b) microvesicles isolated from monocyte supernatants in (a) was measured. Graphs are results are from one donor, showing mean and standard error from 3 wells. Similar results were obtained in cells from 3 donors. TF activity is donor dependent, and, in the absence of LPS stimulation, usually undetectable, but never greater than 1 pg/mL in media only or THC only controls. Grey bars are LPS only, open bars are vehicle control and LPS, solid black bars are THC and LPS. * p < 0.001 via ANOVA followed by Bonferroni post test comparing THC to vehicle. c Whole cell lysates from monocytes were analyzed by western blot for indicated proteins. TF western blots were stripped and reprobed for actin. d, e Total mRNA was isolated from monocytes treated with THC or vehicle 30 min prior to LPS and analyzed for TF and GAPDH by quantitative real-time PCR. d TF versus GAPDH mRNA from one representative donor is graphed. Data is presented as a relative fold change compared to untreated cells. e Mean and standard error from at least 3 donors are graphed relative to vehicle (dotted line at 1) at indicated time points

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