Figure 7
GW9662 does not enhance macrophage phagocytosis of apoptotic cells. (A-B) Apoptotic cells (ACs) were obtained by incubating healthy human neutrophils in 10% FBS-RPMI for 20 hours. CFSE-labeled ACs were added for 30 minutes, at a 5:1 ratio, to CD14-labeled macrophages cultured for 6 days in complete medium in the absence of cytokines (untreated; M0 differentiation) or in the presence of IL-4 (20 ng/ml; M2a differentiation), with or without GW9662 (10 μM). Percentages of total (CFSE+) and highly (CFSEbright) efferocytic macrophages are depicted. (B) Pooled data are represented as mean values ± SEM. Analysis was performed using Student’s paired t-test. *P <0.05. Data are representative of ten independent experiments.