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Figure 4 | Journal of Inflammation

Figure 4

From: The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization

Figure 4

Rosiglitazone attenuates M2c polarization induced by dexamethasone. (A-D) Healthy monocytes were cultured in serum-free medium in the presence or absence of dexamethasone (1 nM; M2c differentiation), with or without the PPAR-γ agonist rosiglitazone (1–100 μM), for 4 days. Expression of MerTK and CD163 was measured by flow cytometry. Vehicle controls (DMSO > 0.15%) are shown in (A). Percentages of cells with normal morphology according to forward (FSC) and side scatter (SSC) and contained in the gate of analysis depicted in (A) are shown in (D). (B-D) Pooled data are represented as mean values ± SEM. Analysis was performed using one-way repeated measures ANOVA with Newman-Keuls multiple comparisons test and Student’s paired t-test. *P < 0.05; **P < 0.01. When not specified by additional graphic signs, statistical annotations (asterisks) refer to comparisons with respect to the relative rosiglitazone untreated control group. Data are representative of three independent experiments.

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