Figure 1

GW9662 inhibits IL-4-driven alternative activation by inducing a phenotypic M2a-to-M2c switch. (A-B) Human monocytes were sorted from healthy PBMCs through negative selection magnetic beads and cultured in serum-free medium in the presence of IL-4 (20 ng/ml; M2a differentiation), with or without the PPAR-γ antagonist GW9662 (0.01-10 μM), for 4 days. Expression of the M2 markers CD206 (mannose receptor), CD209 (DC-SIGN), CD163 and MerTK was measured by flow cytometry. (B) Pooled data are represented as mean values ± SEM. Analysis was performed using one-way repeated measures ANOVA with Newman-Keuls multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001. Data are representative of three independent experiments.