Figure 5
Effect of NF-kappaB pathway inhibitors on MMP-9 induction. THP-1 cells were treated with FSL-1 for different time points and cell lysates were prepared as described in methods. Samples were run on denaturing gels. Phosphorylated IKKα/β, IkB, and NF-κB are depicted in the upper panel and B-actin is shown in the bottom panel (A). THP-1 cells were pretreated with NF-κB inhibitors (BAY 11–7085, 10 μM; Triptolide, 10 μM or Resveratrol, 15 uM) for 1 hr and then treated with HKLM (3x107/ml) for 24 hr. Cells and supernatants were collected. Cells were used for the isolation of total RNA and MMP-9 mRNA was assessed by real-time PCR (B). Secreted levels of MMP-9 protein were determined in supernatants by ELISA (C). Data are shown as mean ± SE. An asterisk (*) represents P-value of <0.05.