Figure 3

Effect of MyD88 deficiency on the induction of MMP-9 by HKLM. THP-1-XBlue™-defMyD cells (Cells deficient in MyD88 activity) were treated with HKLM (3x10⁷/ml), TNF-alpha (25 ng/ml) and vehicle (water; 2ul) for 24 hr. Cells and supernatants were collected. Cells were used for the isolation of total RNA and MMP-9 gene expression was quantified by real-time PCR (A). Secreted levels of MMP-9 protein were determined in supernatants by ELISA (B). SEAP reporter activity (degree of AP-1/NF-κB activation) was also determined in cell supernatants (C). Data are shown as mean ± SE. An asterisk (*) represents P-value of <0.05.