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Figure 1 | Journal of Inflammation

Figure 1

From: TLR2 and AP-1/NF-kappaB are involved in the regulation of MMP-9 elicited by heat killed Listeria monocytogenes in human monocytic THP-1 cells

Figure 1

HKLM up-regulates MMP-9 expression in THP-1 cells. THP-1 cells were stimulated with HKLM different concentrations (1-9x107 particles/ml). Cells and culture supernatants were collected. Total cellular RNA was isolated and MMP-9 mRNA was quantified by real time PCR. Relative mRNA expression was expressed as fold expression over average of gene expression in vehicle-treated cells. The average gene expression level in vehicle-treated cells was assumed to be 1 (A). Secreted MMP-9 levels were measured in supernatants by ELISA (B). THP-1 cells were stimulated with HKLM (3x107/ml), TNF-alphalph (25 ng/ml; positive control) and vehicle (H2O; 2ul/ml) for 24 hrs. Cells and culture supernatants were collected. MMP-9 mRNA was quantified by real-time PCR (D) and secreted levels of TIMP-1 and MMP-9 were measured in the supernatants by ELISA (C & E). SEAP reporter activity (degree of NF-κB /AP-1 activation) was determined in supernatants as described in materials and methods (F). The results obtained from three independent experiments are shown. The data are presented as mean ± SE. An asterisk (*) represents P-value of <0.05.

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