Overexpressing/suppressing ATF4 in endothelial cells promotes/inhibits THP-1 cell adhesion. A). Mouse retinal endothelial cells were transduced with Ad-Ctrl or Ad-ATF4 for 24 h. THP-1 cells were added and co-cultured for 3 hours. Adherent monocytes to endothelial cells were counted from 5 random visual fields. B). Brain endothelial cells isolated from ATF4 +/+ or ATF4−/− mice were challenged with 250 ng/ml LPS for 24 h. THP-1 monocyte adhesion was assessed as described in (A). Images are representatives of 3 independent experiments. Results are expressed as mean ± SD (n = 3). ** P < 0.01 vs. Ad-Ctrl or WT; ++ p < 0.01 vs. WT + LPS.