Figure 1

MSU induced IL-1 β production in FLS. FLS were stimulated with 1 ug/ml, 10 ug/ml, 50 ug/ml, 100 ug/ml, 250 ug/ml and 500 ug/ml of MSU at 6 h, 12 h, 24 h and 48 h respectively. The control group is treated with PBS, which is used to dilute different dosage of MSU. Supernatants were collected for IL-1β, TNF-α and IL-6 protein detection by ELISA. The concentration of MSU 50 ug/ml induced the significant increase of IL-1β in the supernatants collected after 6 hours MSU exposure (A, p<0.05). There is no significant difference for the levels of IL-6 (B) and TNF-α (C) in the supernatant of FLS exposed to different concentration MSU at the four time courses. Data presented are mean ± S.E.M. *represents P < 0.05 in comparison with control during statistical analysis.