Effect of Ara 3000 beta® on IL-1 induced MMP mRNA expression. Human articular chondrocytes were cultured for 6–7 days. Then, they were incubated with IL-1β (1 ng/ml) (IL1) or vehicle (C) in the presence or not of Ara 3000 beta® (dilution 1:250 or 1:1000). At the end of the incubation, MMP mRNA level was evaluated by RT-PCR. A- Cells were co-treated with IL-1 and Ara 3000 beta® for 48 h. B - Cells were incubated with Ara 3000 beta® for 24 h. Then medium was changed and cells were cotreated with IL-1 and Ara 3000 beta® for 24 h.