Table 1 All primers used in this study
From: MicroRNA-181b regulates endotoxin tolerance by targeting IL-6 in macrophage RAW264.7 cells
Name | Sequence |
|---|---|
U6 F | 5′-CTCGCTTCGGCAGCACA-3′ |
U6 R | 5′-AACGCTTCACGAATTTGCGT-3′ |
β-actin F | 5′-AGAGGGAAATCGTGCGTGAC-3′ |
β-actin R | 5′-CAATAGTGATGACCTGGCCGT-3′ |
IL-6 (WT) F | 5′-AAACTAGTGAAATATATCCTGTTGTCAGG-3′ |
IL-6 (WT) R | 5′-GGAAGCTTCCAACATTCATATTGTCAGTTC-3′ |
IL-6 (MUT) F | 5′-AAACTAGTGAAATATATCCTGTTGTCAGG-3′ |
IL-6 (MUT) R | 5′-GGAAGCTTCCTAGAGTAACATTGTCAGTTC-3′ |
IL-6 F | 5′-AACGATGATGCACTTGCAGA-3′ |
IL-6 R | 5′-GAGCATTGGAAATTGGGGTA-3′ |
P65 F | 5′-ATGGCTACTATGAGGCTGACC-3′ |
P65 R | 5′-GCTGGCTAATGGCTTGCT-3′ |
pri-miR-181b F | 5′-CAGCACATCTCTGCCTCACA-3′ |
pri-miR-181b R | 5′-TTGCGGTTCTGTCTTCAGC-3′ |
miR-181b RT | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAACCCAC-3′ |
miR-181b F | 5′-AAAACATTCATTGCTGTCG-3′ |
miR-181a RT | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAACTCAC-3′ |
miR-181a F | 5′-TGAACATTCAACGCTGTCG-3′ |
miR-181c RT | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAACTCAC-3′ |
miR-181c F | 5′-GTAACATTCAACCTGTCG-3′ |
let-7 g RT | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAAACTGT-3′ |
let-7 g F | 5′-GATCTGAGGTAGTAGTTTGT-3′ |
miRNA Universal R | 5′-GTGCAGGGTCCGAGGT-3′ |
ChIP F | 5′-TTTCTGGTGGCTCTTGATT-3′ |
ChIP R | 5′-ACCTTTCCTCGGCTTTCTA-3′ |