Figure 4

miR-181b inhibitors abrogates LPS-induced IL-6 tolerance. (A) Effects of TSA on the endogenous IL-6 protein levels during LPS tolerance. RAW264.7 cells were primed with 100 ng/ml LPS continuously for 24 h and washed twice with PBS, then cells were primed with 1 μM TSA and 100 ng/ml LPS. Another 6 h and 12 h later, IL-6 were analyzed by ELISA. cells were primed with 100 ng/ml LPS continuously for 24 h and washed twice with PBS, then cells were transfected with miR-181b inhibitors and primed with 100 ng/ml LPS. 6 h and 12 h later, miR-181b were analyzed by qRT-PCR (B) and IL-6 were analyzed by ELISA (C). NC: negative control; I: inhibitors. The data were subjected to Student’s t-test. **p < 0.01, ***p < 0.001. (B, Ccp Effects of miR-181b inhibitors on the endogenous IL-6 protein levels during LPS tolerance. RAW264.7.