Figure 1

CLA primes monocytes to an anti-inflammatory MΦ2 macrophage phenotype. RT-PCR analysis of (a) CD14 and CD68 and (b) CD163 and MR mRNA expression in or M-CSF stimulated (100 ng/ml) differentiating HPBMCs following treatment with c-9,t-11-CLA, t-10,c-12-CLA, CLA blend (80:20 c-9,t-11:t-10,c-12), OA, LA or TROG. In unstimulated conditions, both CLA isomers and their blend decrease CD14 expression and increase expression of both CD163 and MR, without affecting CD68 expression. Following M-CSF stimulation, c-9,t-11-CLA and CLA blend decrease the mature macrophage marker CD68 and increase expression of both CD163 and MR (MΦ2 markers). Data are mean +/− SEM of three independent experiments. Data is expressed as fold change in gene expression relative to DMSO control, where *p < 0.05; ** p < 0.01 and ***p < 0.001 vs DMSO.