Figure 3

NR4A receptors are required for PGE ₂ induction of PRL. A. Triplicate wells of K4IM synoviocytes were left untreated or stimulated with PGE₂ (1 μM) in serum-free media for the times indicated. NR4A2 expression levels were measured by RT-qPCR. *p < 0.05. B. PRL expression levels were measured by RT-qPCR in the same experiment. *p < 0.05, **p < 0.005. C. Scrambled shRNA or NR4A1-3 shRNA transduced K4IM synoviocytes were left untreated or treated with PGE₂ for one hour. NR4A2 expression levels were measured by RT-qPCR. **p < 0.005. D. Scrambled shRNA or NR4A1-3 shRNA transduced K4IM synoviocytes were left untreated or treated with PGE₂ for 24 hours. PRL expression levels were measured by RT-qPCR. **p < 0.005.