Depleting NKX3.1 results in alterations in the expression of genes involved in the regulation of oxidative stress. A. Time course (3, 6 and 24 h) of CM exposure influences gene expression in LNCaP cells. Pink and green colors indicate the gene expression of pro- and anti-oxidant enzymes, respectively. B. The changes in gene expression caused by NKX3.1 depletion correlate with the changes that occurred after CM treatment (250 pg/ml of TNFα for 24 h) in LNCaP cells. C. Histogram plot demonstrates the genes that underwent changes in expression upon NKX3.1 depletion that did not correlate with CM exposure. Red and green bars indicate upregulation and downregulation, respectively. Western blot confirms the depleted NKX3.1 protein level in comparison to B-actin expression due to NKX3.1 silencing.