Figure 6

Effect of polyphenol-rich plant extracts on the viability and ROS production of preadipocytes exposed to H ₂ O ₂ . (a) Cells were exposed to each plant extract (25 μM GAE) or treated with H₂O₂ (200 μM) in the presence or not of each plant extract (25 μM GAE) for 24 h. Then, the mitochondrial metabolic activity of cells was measured by MTT assay. (b) Cells were exposed to 10 μM of DCFH-DA for 45 min at 37°C and then were treated with each plant extract (25 μM GAE) or caffeic acid as a positive control (25 μM), or treated with H₂O₂ (200 μM) in the presence or not of each plant extract (25 μM GAE) or caffeic acid (25 μM) for 1 h. Data are means ± SEM of three independent experiments. ***: p < 0.001, ****: p < 0.0001 as compared to H₂O₂.