Figure 5

Silencing CRMP2 promoted macrophage polarization to M2 in the wound of ApoE ^−/− mice with MI. A. Representative confocal microscopy images showed marked decrease in the expression of CRMP2 in the infiltrated macrophages, in the population of M1 (CD86 positive cells), and in the increase in the population of M2 (CD206 positive cells) in the siCRMP2-treated ApoE^−/− mice 3 days after MI. Scale bar indicates 50 μm B-E. Quantitation of CRMP2⁺ macrophages (CD11b⁺ and CRMP2⁺), total macrophages (CD11b⁺), M1 macrophages (CD86⁺), and M2 macrophages (CD206⁺) in the wound 3, 7 and 10 days after MI in ApoE^−/− mice. n = 4 mice per group and n = 4 high power fields (HPF). Data are mean ± SEM; **p < 0.01 compared to control.