Figure 4

CRMP2 was predominantly expressed in M1 but not M2 macrophages infiltrated in the wound of wildtype and ApoE ^−/− mice with MI. A & B. Representative confocal microscopy images showed the predominance of M1 (CD11b⁺, iNOS⁺) macrophages 3 days after MI in both wildtype and ApoE^−/− mice with MI, and predominance of M2 (CD11b⁺, IGF-1⁺) macrophages in wildtype but predominance of M2 (CD11b⁺, IGF-1⁺) macrophages in ApoE^−/− mice 10 days after MI. Scale bar indicates 50 μm. C. Quantitation of M1 (CD11b⁺, iNOS⁺) macrophages in the wound 3 and 10 days after MI. D. Quantitation of M2 (CD11b⁺, IGF-1⁺) macrophages in the wound 3 and 10 days after MI. E. Representative confocal microscopy images showed the expression of CRMP2 in the infiltrated macrophages 3 days after MI in ApoE^−/− mice. Arrows indicated localization of CRMP2 in one pole outside of the nucleus. F. Representative confocal microscopy images showed the expression of CRMP2 in the infiltrated macrophages 3 days after MI in WT mice. G. Quantitation of CRMP2 positive macrophages in the wound 3 and 10 days after MI in WT and ApoE^−/− mice. In all of the quantitative experiments, n = 4 mice per group and n = 4 high power fields (hpf). Data are mean ± SEM. *P < 0.05 compared to WT control.