Expression of CRMP2 in BMDM subsets. A. BMDMs were incubated with IFN-γ + LPS (M1), IL-4 (M2a), IC+ LPS (M2b), or IL-10 (M2c) for 7 h. The mRNA levels of iNOS, IL12p40, Arg-1, and CD163 genes were detected by qPCR. Data mean ± SEM of gene fold changes against untreated M0 cells. ***P < 0.001 vs. untreated M0 cells. B. Western blot analysis of CRMP2 with a specific antibody showed higher level of CRMP2 expression in M1 versus M2 and M0 macrophage subsets. The immunoblots of β-actin was used as a loading control. Shown are representative data from three independent experiments with similar results.