L- and D, L-SFN do not show consistent significant differences between their effects on MDP-induced NF-
B activity. NOD2 WT cells were treated for 24 h with 0.2 μg/ml MDP in the presence or absence of L- or D, L-SFN (0.5, 2, 5 or 10 μM). Media was collected and added to QUANTI-Blue™ solution and left at room temperature for 40–60 min before being measured using a spectrophotometer at 650 nm. Data shown = mean ± SEM, n = 3. *p < 0.05.