The expression of GR isoforms induced by IL-1β with different time length in vitro. A represents Western blot results of IL-1β-induced protein expression of GR isoforms. Lanes 1-4 represent the expression in sample groups treated with IL-1β-treated for 0 h, 10 h, 20 h and 30 h respectively. The GRα and GRβ expression increased with longer IL-1β-inducing time. B represents the densitometry value (Western blot bands in 2A) ratio of GR isoforms and β-actin. The protein expression of GRα and GRβ increased as the IL-1β-inducing time was increased, and the GRβ expression increased more significantly than the GRα expression. C represents the ratio of GR isoforms mRNA between IL-1β-induced groups and the control group (0 h). The mRNA expression of GRα and GRβ increased as the IL-1β-inducing time was increased, and the GRβ expression increased more significantly than the GRα expression. D represents the GRα/GRβ mRNA expression (measured by FQ-RT-PCR) ratio induced by IL-1β. The GRα/GRβ ratio decreased with the increase of IL-1β-inducing time in nasal polyp tissue. The data presented are means ± SD of three independent experiments with similar trend.