Figure 6
From: The histone deacetylase Hdac1 regulates inflammatory signalling in intestinal epithelial cells
Hdac1 depletion alters RNA polymerase II enrichment at the Ccl2 promoter in IEC-6 cells. Chromatin immunoprecipitation assays were performed with chromatin extracts from shCtrl and shHDAC1 IEC-6 cells, without antibody (−), or with antibodies against RNA polymerase II (+). Input and immunoprecipitated samples were subjected to semi-quantitative PCR analysis with oligonucleotides amplifying Ccl2 and Gapdh promoter (prom) and downstream gene body (ex 2) sequences. Results from two independent experiments are shown.