Figure 3
From: The histone deacetylase Hdac1 regulates inflammatory signalling in intestinal epithelial cells
Hdac1 depletion differentially modulates the expression of inflammatory response genes in IEC-6 cells. Two shCtrl (5; 9) and shHDAC1 (18; 21) IEC-6 cell lines were treated with or without 10 ng/ml of IL-1β for 24 h. Total RNAs were isolated and expression levels of various inflammatory response genes (Hp, Kng1, Ccl2, Ccl5, Cxcl1, C3 and Cxcl2) were assessed by semi-quantitative RT-PCR. Gapdh was used as a control for the amount of cDNA amplified. Results are representative of two independent experiments.