Figure 4

Vocal fold fibroblasts NF-κB translocation into the nucleus. Normal, scar, and polyp VFF were seeded on cover slips at 1.5 × 10⁵ cells and stimulated with or without 5 μg/ml of LPS for 30 min (A) or 45 min (B). Cells were stained for NF-κB p65 translocation into the nucleus and nucleic acids. Data is graphed as the mean of the expression % of total cells counted ± SD. Letters represent a statistical significance of p < 0.05 when comparing (a) T21 (b) T59 (c) scar (d) polyp across similar conditions. T represents differences between 30 and 45 min time points compared within treatment groups. *p < 0.05 represent differences within cell type with (w/) LPS compared to without (w/o) LPS within each cell type.