The combination of red palm oil and rooibos show anti-inflammatory effects in rats

Journal of Inflammation

Table 2 Study design illustrating the experimental groups and study protocol

	NO-LPS				LPS
Groups	Control	Rooibos	RPO	RB + RPO	Control	Rooibos	RPO	RB + RPO
Feeding time	28 days	28 days	28 days	28 days	28 days	28 days	28 days	28 days
Treatment	PBS	PBS	PBS	PBS	LPS	LPS	LPS	LPS
	*Heart excision and perfusion protocol				*Heart excision and perfusion protocol

RB: rooibos.
RPO: red palm oil.
LPS: liposaccharide.
Treatment: 0.5 mg/kg LPS was injected intraperitoneally to induce inflammation while 0.1 ml PBS was injected as a vehicle in control groups 18 hours prior to sacrificing.
^*Hearts were excised and perfused for 20 minutes in a Langendorrf mode, baseline heart function was recorded at 20 minutes after which hearts were freeze clamped for cytokine analysis.

ISSN: 1476-9255